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adenylate kinase may lead to conversion of ADP into ATP and AMP and thereby confound interpretation of the ADP/ATP specificity experiments. Solving the conundrum of how some twin-specificity archaeal ligases may make the most of ATP and NAD+ will count on capturing crystal buildings of such enzymes with Each and every nucleotide certain.
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An engineered step 3 arrest mutant of DNA ligase would, if broadly Energetic, pepper the genome with unresected 5′-adenylates at sites of DNA mend or joining of Okazaki fragments. This is able to be catastrophic if the extent of DNA five′-adenylylation exceeds the mobile's ability to get rid of the lesions (33). This type of circumstance is analogous to “poisoning” of topoisomerase IB by camptothecin medicine or drug-mimetic enzyme mutations that selectively impede the DNA ligation action with the topoisomerase pathway.
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